The Koopalings are listed below in order from what is possibly, but not confirmed, to be their birth orders from oldest to youngest:
- Ludwig von Koopa - oldest
- Lemmy Koopa - second oldest
- Roy Koopa - third oldest
- Iggy Koopa - middle child
- Wendy O. Koopa - third youngest
- Morton Koopa Jr. - second youngest
- Larry Koopa - youngest
If there is something ambiguous about the Koopalings is their birth order. According to the Player's Guide for Super Mario Bros. 3, Larry is stated to be the youngest of Bowser's children, while Ludwig is said to be the eldest. As Larry is the first Koopaling of the game, while Ludwig is the last, it is strongly implied that all seven Koopalings are fought in order from youngest to oldest:(Although the implied birth order does not fit appearance and personality of some Koopaings.) Larry Koopa, Morton Koopa Jr, Wendy O. Koopa, Iggy Koopa, Roy Koopa, Lemmy Koopa, Ludwig von Koopa. However,the birth order for the five middle children has never been officially confirmed. The release of New Super Mario Bros. Wii made even more confusion about their birth order. Morton is supposedly the second youngest Koopaling. However, he is very large in size and has a low-pitched voice, making him appear like one of the oldest Koopalings. As of now, however, the true birth order of the Koopalings remains to be seen.
In the Super Mario Bros. 3 Cartoon, an alternate birth order was used. Bully (Roy Koopa) is regarded as the eldest, while Hip and Hop (Lemmy and Iggy Koopa) are six-years-old twins, as well as the youngest. However, the cartoons are considered non-canonical.